Mawu Oyamba

M'ma laboratories amakono, machitidwe olondola amapangitsa kuti ziwiya ziziwonjezeka. Makamaka akamagwira ntchito ndi kuchuluka kwa zakumwa, ogwira ntchito nthawi zambiri amakumana ndi zovuta zambiri. Ma labware achikale, ngakhale akadali ofunikira pamachitidwe anthawi zonse, amakhala ochulukira komanso osalondola pogwira zinthu zazing'ono zamadzimadzi, zomwe zimapangitsa kuti zikhale zovuta kukwaniritsa zofunikira pakulondola komanso ukhondo pamayesero.

Mapangidwe apamwamba kwambiri a botolo laling'ono lomaliza maphunziro amadzimadzi amapangitsa kuti madzi azitha kuwongolera komanso odalirika.

Chifukwa chiyani Labu sangachite popanda Mabotolo Aang'ono Omaliza Maphunziro a Burette?

Mabotolo ang'onoang'ono omaliza maphunziro a burette amagwiritsidwa ntchito kwambiri m'malo opangira ma labotale chifukwa amawonetsa maubwino apadera potengera kulondola, chitetezo komanso magwiridwe antchito.

1. Kuthekera koyezera kolondola

Kubwerezabwereza ndi kulondola kwa zoyesera zimadalira kuwonjezereka kwamadzimadzi. Mabotolo apadera a dropper amakhala ndi zolakwika zazing'ono kuposa zotengera zachikhalidwe ndipo amatha kukwaniritsa zofunikira pakuyesa ndi zowonjezera zowongoleredwa mwamphamvu.

2. Anti-kuipitsa mapangidwe

Botolo la dropper limapangidwa ndi kapu ya screw-seal kapena nsonga yotsitsa imodzi, yomwe imathandizira kwambiri kusindikiza ndikuletsa zomwe zili mkati kuti zisatuluke kapena kutulutsa okosijeni. Panthawi imodzimodziyo, poyerekeza ndi ntchito za pipette zomwe zimafuna kusintha nsonga pafupipafupi, nsonga ya dropper palokha imapewa ntchito zambiri ndipo imachepetsa kuthekera kwa kuipitsidwa, kuwongolera bwino komanso kusunga zinthu zomwe zimagwiritsidwa ntchito.

3. Chitetezo chakuthupi

Mabotolo a dropper omaliza maphunziro omwe timagulitsa amapangidwa ndi galasi lapamwamba la borosilicate, lopanda kutentha komanso lopanda dzimbiri, oyenera kuchiritsa kutentha kwambiri kapena asidi amphamvu ndi ma reagents a alkali.

Zochitika Zofananira za Ntchito

Mabotolo ang'onoang'ono omaliza maphunziro a burette amagwiritsidwa ntchito kwambiri m'magawo ambiri oyesera chifukwa chochita bwino komanso kusinthasintha, makamaka pantchito zoyesera zomwe zimafuna kuwongolera kwamadzimadzi komanso kugwira ntchito mosavuta.

1. Kuyesera kwa mamolekyulu a biology

Mu ntchito maselo mlingo, misa ndi kuchuluka kwa reagents mwachindunji zotsatira za kuyesera. Mabotolo a Dropper ndi abwino kwa DNA / RNA m'zigawo ndi kusungidwa, ndipo kukula kwa 1ml kumalepheretsa kusungunuka kwachitsanzo komanso kumathandizira kusungirako mufiriji. Pochita ma enzyme kapena ma antibody, mabotolo a 3ml angagwiritsidwe ntchito kuperekera kuchuluka kwa ma reagents, kupewa kutayika kwa ntchito komwe kumachitika chifukwa cha kuzizira kobwerezabwereza komanso kusungunuka kwa mabotolo akulu, ndikuwonetsetsa kubweza ndi kukhazikika kwa zoyeserera.

2. Kusanthula mankhwala

Pakukonzekereratu vial pakuwunika kuchuluka, botolo la dropper la 5 ml limapereka mpata wowonera mosavuta komanso kuwongolera ndipo ndiloyenera kuchepetsedwa kwamagawo angapo. Pazinthu zina zapoizoni kwambiri kapena zosasunthika, nsonga yodontha podontha ya botolo ndi kapangidwe ka ulusi womata kumathandizira kwambiri chitetezo komanso kumachepetsa chiwopsezo cha kuwonekera kwa ogwira ntchito ndi mpweya wa mpweya.

3. Ma laboratories ophunzitsa

M'makoleji ndi sekondale zasayansi kuphunzitsa, pasadakhale reagent dispensing osati mogwira kuchepetsa reagent zinyalala, komanso kuchepetsa mwayi wophunzira kukhudzana mwachindunji ndi mankhwala oopsa ndi kusintha khalidwe la maphunziro chitetezo. Mabotolo owonekera okhala ndi masikelo amathandizira ophunzira kukhazikitsa chidziwitso cha "volume perception" ndi "precise titration", komanso kupititsa patsogolo maphunziro a luso loyesera.

Kalozera Wosankha

Pakati pa mitundu ndi zida zambiri zomwe mungasankhe, kugula kwasayansi komanso mwanzeru mabotolo ang'onoang'ono omaliza maphunziro ndikofunikira kuti zitsimikizire kudalirika komanso chitetezo chazotsatira zoyeserera.

1. Kusankha luso

Zofunikira zoyeserera za Opaque zimatengera kukula kwa botolo:

• 1ml/2mlmabotolo ndi oyenera reagents ang'onoang'ono-mtengo umodzi, kuchepetsa zinyalala ndi kutsogolera kusungirako.
• 3ml kuMabotolo ndi omwe amapezeka kwambiri komanso kukula konsekonse, oyenera kuyesa kwatsiku ndi tsiku pakugawa kwamadzimadzi, mphamvu zolimbitsa thupi komanso zosavuta kunyamula.
• 5ml kumabotolo ndi oyenera kuyankha pafupipafupi, kupewa kuwonjezeredwa mobwerezabwereza ndikuwongolera magwiridwe antchito a zoyeserera.

2. Chofunikira kwambiri pa parameter

Njira yosankha iyenera kuyang'ana pa:

• Kumveka bwino kwa sikelo: Mabotolo otsitsa apamwamba kwambiri amayenera kukhala okhazikika kapena osindikizidwa ndi zomatira kwambiri kuti asafooke pakuchotsa kutentha kwambiri kapena kuyeretsa komanso kutsimikizira kuwerengeka kwanthawi yayitali.
• Kusindikiza: Ndibwino kuti muyese kuyesa kosavuta musanagule koyamba - mudzaze botolo ndi madzi, pukutani kapu mwamphamvu ndikuyitembenuza kwa maola 24 kuti muwone ngati pali vuto lililonse lotayirira, lomwe limagwiritsidwa ntchito kuyerekezera momwe zinthu zimasungira.

3. Chenjezo lopewa mbuna

Malo a labotale amafunikira kwambiri paziwiya, ndipo zotsatirazi ziyenera kuwonetseredwa:

• Mabotolo apulasitiki osakhala bwino amatha kukhala ndi mapulasitiki kapena organic solvent leachate, makamaka posunga ma acid kapena organic reagents, omwe amatha kuipitsidwa, zomwe zimakhudza chiyero ndi chitetezo cha zoyeserera.
• Zogulitsa zotsika mtengo zokhala ndi zolakwika zazikulu zitha kupangitsa kuti pakhale kuchuluka kolakwika, komwe kungayambitse kukondera koyesera kapena kulephera kubwereza, makamaka popanga zinthu zomwe zimakhudzidwa ndi chidwi.

Mapeto

Mabotolo ang'onoang'ono omaliza maphunziro ndi osawoneka bwino koma amagwira ntchito yofunika kwambiri m'malo ovuta komanso abwino a labotale. Kupyolera mu kuwongolera sikelo yolondola/kusindikiza kwabwinoko ndi zida zomwe amakonda zogwirizanirana ndi mankhwala, amapereka chitsimikizo cha katatu cha "kulondola + chitetezo + chogwira ntchito" poyeserera. Zida zofunika izi koma zofunikira zimatsimikizira kudalirika kwa deta, kukhulupirika kwa zitsanzo, ndi kubwerezabwereza kwa njira zoyesera.

Oyesera asankhe mphamvu ndi zinthu za mabotolo molingana ndi ntchito zosiyanasiyana zoyesera kuti athe kupititsa patsogolo magwiridwe antchito ndikupewa zolakwika kapena zoopsa zosafunikira. Vial yofananira bwino ikhoza kukhala gawo lofunikira pakupambana kwa kuyesa.