Chiyambi

Mu ma laboratories amakono, ntchito zolondola zimawonjezera kufunikira kwa ziwiya. Makamaka akamagwiritsa ntchito madzi ochepa, ogwiritsa ntchito nthawi zambiri amakumana ndi mavuto ambiri. Ma labware achikhalidwe, ngakhale kuti akadali ofunika kwambiri pa ntchito zachizolowezi, ndi olemera komanso osalondola pogwira madzi ochepa, zomwe zimapangitsa kuti zikhale zovuta kukwaniritsa zofunikira zolimba kuti zikhale zolondola komanso zaukhondo pazochitika zoyeserera.

Kapangidwe kabwino kwambiri ka botolo laling'ono lokhala ndi madontho ochepa opangidwa bwino kamapangitsa kuti kugawa madzi kukhale kosavuta komanso kodalirika.

N’chifukwa chiyani Labolabu singathe kuchita popanda Mabotolo Ang'onoang'ono Okhala ndi Maphunziro Apamwamba?

Mabotolo ang'onoang'ono a freette opangidwa ndi pulasitiki amagwiritsidwa ntchito kwambiri m'ma laboratories chifukwa ali ndi ubwino wapadera pankhani ya kulondola, chitetezo, komanso kugwira ntchito bwino.

1. Kutha kuyeza molondola

Kubwerezabwereza ndi kulondola kwa zoyeserera kumadalira kuwonjezera madzi molondola. Mabotolo apadera ochotsera madzi amakhala ndi vuto lochepa kuposa zotengera zachikhalidwe ndipo amatha kukwaniritsa zofunikira za zoyeserera ndi zowonjezera zotsalira zomwe zimawongoleredwa bwino.

2. Kapangidwe koletsa kuipitsa

Botolo la dropper limapangidwa ndi chivundikiro chotseka ndi screw kapena nsonga ya dropper ya chidutswa chimodzi, zomwe zimapangitsa kuti kutseka kukhale bwino komanso kuti zomwe zili mkati mwake zisatuluke kapena kusungunuka. Nthawi yomweyo, poyerekeza ndi ntchito za pipette zomwe zimafuna kusintha nsonga pafupipafupi, nsonga ya dropper yokha imapewa ntchito zingapo ndipo imachepetsa mwayi woti zinthu ziwonongeke, kukonza magwiridwe antchito ndikusunga zinthu zogwiritsidwa ntchito.

3. Chitetezo cha zinthu

Mabotolo odulira omwe timagulitsa amapangidwa ndi galasi lolimba kwambiri la borosilicate, lolimba kutentha komanso lolimba dzimbiri, loyenera kutsukidwa kutentha kwambiri kapena zinthu zamphamvu za asidi ndi alkali.

Zochitika Zachizolowezi Zogwiritsira Ntchito

Mabotolo ang'onoang'ono a freette omwe ali ndi voliyumu yambiri amagwiritsidwa ntchito kwambiri m'magawo ambiri oyesera chifukwa cha magwiridwe antchito awo komanso kusinthasintha kwawo, makamaka pantchito zoyesera zomwe zimafuna kulamulira bwino madzi komanso kugwiritsa ntchito mosavuta.

1. Kuyesera kwa zamoyo zamaselo

Mu ntchito za molekyulu, kulemera ndi kuchuluka kwa ma reagents zimakhudza mwachindunji zotsatira za kuyesera. Mabotolo otsitsa ndi abwino kwambiri pochotsa ndi kusunga DNA/RNA, ndipo kukula kwa 1ml kumaletsa bwino kuuluka kwa zitsanzo ndipo kumathandiza kusungira mufiriji. Mu ntchito za enzyme kapena antibody, mabotolo a 3ml angagwiritsidwe ntchito kupereka kuchuluka koyenera kwa ma reagents, kupewa kutayika kwa ntchito komwe kumachitika chifukwa cha kuzizira mobwerezabwereza ndi kusungunuka kwa mabotolo akuluakulu, ndikuwonetsetsa kuti zoyesererazo ziberekanso komanso kukhazikika.

2. Kusanthula mankhwala

Pokonzekera botolo la 5 ml poyesa kuchuluka kwa zinthu, botolo la dropper la 5 ml limapereka malo oti liziwoneka mosavuta komanso kugwiritsidwa ntchito mosavuta ndipo ndi loyenera kuchepetsedwa m'magawo ambiri. Pa zinthu zina zoopsa kwambiri kapena zosakhazikika, kapangidwe ka ulusi wotseka botolo kamene kamateteza kutayikira kwa madzi komanso kuchepetsa chiopsezo cha kukhudzana ndi anthu komanso kupangika kwa mpweya.

3. Malo ophunzitsira

Mu makoleji ndi masukulu a sekondale kuphunzitsa, kupereka mankhwala ophera tizilombo toyambitsa matenda pasadakhale sikungochepetsa zinyalala za mankhwala ophera tizilombo, komanso kuchepetsa mwayi woti ophunzira akhudzidwe mwachindunji ndi mankhwala oopsa ndikupititsa patsogolo maphunziro achitetezo. Mabotolo owonekera bwino okhala ndi sikelo amathandiza ophunzira kuzindikira "kuzindikira kuchuluka kwa madzi" ndi "kuzindikira bwino momwe madzi amagwirira ntchito", ndikuwonjezera maphunziro a luso loyesera.

Buku Lotsogolera Kusankha

Pakati pa mitundu ndi zipangizo zambiri zoti musankhe, kugula mabotolo ang'onoang'ono opangidwa ndi dropper m'njira yasayansi komanso mwanzeru ndikofunikira kuti zitsimikizire kudalirika ndi chitetezo cha zotsatira za kafukufuku.

1. Nzeru yosankha mphamvu

Zofunikira zoyeserera zosawoneka bwino zimatengera kukula kwa mabotolo osinthidwa:

• 1ml/2mlMabotolowa ndi oyenera kugwiritsa ntchito zinthu zazing'ono kwambiri zogwiritsidwa ntchito pa chinthu chimodzi, zomwe zimachepetsa zinyalala komanso zimathandiza kusunga zinthu mosavuta.
• 3mlMabotolo ndi ofala kwambiri komanso odziwika bwino, oyenera kugwiritsidwa ntchito tsiku ndi tsiku popereka madzi, mphamvu yake ndi yosavuta kunyamula.
• 5mlMabotolowa ndi oyenera kugwiritsidwa ntchito pafupipafupi, kupewa kudzazanso mobwerezabwereza komanso kukonza magwiridwe antchito a zoyeserera.

2. Kuyang'ana kwambiri pazigawo zazikulu

Njira yosankha iyenera kuyang'ana kwambiri pa:

• Kumveka bwino kwa sikeloMabotolo abwino kwambiri ochotsera madontho ayenera kujambulidwa ndi laser kapena kusindikizidwa ndi kumatira kwambiri kuti sikelo isafe kutentha kwambiri kapena kutsukidwa komanso kuti zitsimikizidwe kuti ziwerengedwa kwa nthawi yayitali.
• Kutseka: Ndikofunikira kuchita kafukufuku wosavuta wokhudza kusintha kwa zinthu musanagule koyamba - dzazani botolo ndi madzi, gwirani chivundikirocho mwamphamvu ndikuchitembenuza kwa maola 24 kuti muwone ngati pali vuto lililonse lotayikira, lomwe limagwiritsidwa ntchito kutsanzira momwe zinthu zilili posungira.

3. Chenjezo lopewa misampha

Malo ochitira kafukufuku wa zasayansi amaika kufunika kwakukulu kwa zipangizo zosungiramo zinthu, ndipo nkhani zotsatirazi ziyenera kuganiziridwa bwino:

• Mabotolo apulasitiki osalimba bwino akhoza kukhala ndi mapulasitiki kapena leachate ya organic solvent, makamaka akamasunga ma acid kapena ma organic reagents, omwe angayambitse kuipitsidwa, zomwe zingakhudze chiyero ndi chitetezo cha zoyeserera.
• Zogulitsa zotsika mtengo zokhala ndi zolakwika zazikulu zingayambitse kuchuluka kwa ma spiking kolakwika, zomwe zingayambitse tsankho loyesera kapena kulephera kubwerezabwereza, makamaka popanga machitidwe omwe amakhudzidwa ndi kukhudzika kwa zinthu.

Mapeto

Mabotolo ang'onoang'ono opangidwa ndi ma dropper opangidwa ndi ma ultra-degradable dropper saonekera koma amagwira ntchito yofunika kwambiri m'malo ovuta komanso ogwira ntchito bwino a labotale. Kudzera mu kuwongolera bwino sikelo/kugwira ntchito bwino kwambiri kotseka komanso zipangizo zomwe zimagwirizana ndi mankhwala, amapereka chitsimikizo cha katatu cha "kulondola + chitetezo + kugwira ntchito bwino" pa ntchito zoyesera. Zipangizo zofunika koma zofunika kwambirizi zimatsimikizira kudalirika kwa deta, kukhulupirika kwa zitsanzo, komanso kubwerezabwereza kwa njira zoyesera.

Oyesa ayenera kusankha mphamvu ndi zipangizo za mabotolo moyenera malinga ndi ntchito zosiyanasiyana zoyesera kuti awonjezere magwiridwe antchito ndikupewa zolakwika kapena zoopsa zosafunikira. Botolo lofanana bwino lingakhale gawo lofunika kwambiri pa kupambana kwa kuyesaku.