Chiyambi

Machubu opangidwa ndi ulusi wotayidwa omwe amatayidwa amagwira ntchito yofunika kwambiri pa ntchito za labotale.Kugwiritsa ntchito kwawo moyenera sikuti kumangoteteza kuipitsidwa kwa zitsanzo, kuipitsidwa ndi kutayika kwa zitsanzo, komanso kumaonetsetsa kuti deta yoyesera ndi yolondola. Chifukwa chake, ndikofunikira kwambiri kudziwa bwino njira yogwiritsira ntchito kuti zitsimikizire kuti mayesowo ndi abwino.

Malangizo awa akugwiritsidwa ntchito pa njira zogwiritsira ntchito machubu otukula ulusi wa screw omwe amagwiritsidwa ntchito popanga maselo, kuyesa kwa microbiology, kuyesa zachipatala ndi zina.

Kukonzekera musanagwiritse ntchito

Kukonzekera koyenera musanayesere ndi gawo lofunika kwambiri pakuonetsetsa kuti machubu opangidwa ndi ulusi wotayidwa akuyenda bwino. Choyamba, kulimba kwa phukusi lakunja kuyenera kuyang'aniridwa mosamala ngati pali kusweka kapena kulephera kwa chisindikizo, zomwe ndizofunikira kuti machubuwo asawonongeke. Machubu opakidwa bwino ayenera kusungidwa pamalo ouma komanso oyera kuti apewe chinyezi kapena kuipitsidwa.

Kuyeretsa sikuyenera kunyalanyazidwa. Machubu opangidwa ndi opanga nthawi zonse omwe amatayidwa nthawi zambiri amayeretsedwa ndi gamma irradiation kapena ethylene oxide, ndipo phukusili liyenera kukhala ndi chizindikiro choyera cha kuyeretsedwa komanso tsiku lotha ntchito. Ogwira ntchito ku labotale ayenera kuyang'ana njira yoyeretsedwa ndi tsiku lotha ntchito kuti atsimikizire kuti machubuwo ali bwino kwambiri kuti agwiritsidwe ntchito.

Zinthu zotsatirazi ziyenera kuganiziridwa posankha zofunikira:

1. Kusankha voliyumuMachubu okhazikika a 15ml angasankhidwe kuti agwiritsidwe ntchito poyesa nthawi zonse, pomwe kukula kwa 50ml kumalimbikitsidwa kuti agwiritsidwe ntchito polima m'njira zazikulu.
2. Makhalidwe a zinthu: Zinthu za polypropylene sizimatentha kwambiri, ndizoyenera kuyeretsa kutentha kwambiri; zinthu za polystyrene zimakhala zowonekera bwino, zosavuta kuziona.
3. Zofunikira zapadera: Pa zoyeserera zapadera, monga kusungirako kutentha kochepa, muyenera kusankha zipangizo zopirira kutentha kochepa.

Ogwira ntchito m'ma laboratories ayenera kusankha chitsanzo choyenera kwambiri cha chubu chokulirapo malinga ndi mikhalidwe yeniyeni yoyesera, poganizira zofunikira pa mphamvu ya centrifugal, kuyanjana kwa mankhwala ndi zinthu zina. Ndikofunikira kukhazikitsa njira yogwiritsira ntchito yosankha zinthu zogwiritsidwa ntchito m'ma laboratories kuti zitsimikizire kuti zoyesererazo zikugwirizana komanso zimabwerezedwanso.

Njira Yolondola

1. Kutsegula zinthu

• Tsegulani machubu osungiramo zinthu pamalo oyera ogwirira ntchito kuti muwonetsetse kuti palibe kuipitsidwa kwakunja panthawi yogwiritsa ntchito.
• Valani magolovesi osagwiritsidwa ntchito kapena gwiritsani ntchito ma tweezers osagwiritsidwa ntchito kuti muchotse machubu mukatsegula kuti mupewe kukhudzana mwachindunji ndi malo ofunikira.

2. Ntchito yopangira spike

• Ndikoletsedwa kukhudza khoma lamkati la denga kapena chophimba chamkati cha chivindikiro panthawi yodzaza kuti tipewe kufalikira kwa zinthu zodetsa.
• Kuchuluka kwa madzi kuyenera kulamulidwa powonjezera zitsanzo zamadzimadzi ndipo sayenera kupitirira muyeso waukulu kuti apewe kutaya kapena kutseka bwino zitsanzo panthawi yogwira ntchito.

3. Njira yotsekera

• Chivundikiro cha screw chiyenera kulimba pambuyo powonjezera chitsanzo kuti zitsimikizire kuti chatsekedwa bwino. Kutsekako kungatsimikizidwe mwa kupangitsa kuti pang'onopang'ono kuwone ngati pali kutayikira kulikonse.
• Samalani ndi mphamvu yocheperako yokulungira kuti mupewe mphamvu yochulukirapo yomwe ingachititse kuti ulusi uwonongeke kapena kusweka, zomwe zingakhudze kugwiritsidwanso ntchito kapena kutseka.

4. Kulemba ndi kulemba

• Gwiritsani ntchito zilembo kapena zolembera za labu zosasungunuka, zosalowa madzi, zosaboola kuti mulembe molondola zambiri za zitsanzo pa malo oyera komanso ouma a chubu.
• Pewani kugwiritsa ntchito mapepala olembera kapena zolembera za inki zomwe zimatha kutha chifukwa cha chinyezi kuti musataye zambiri panthawi yosungira.

Malangizo Ogwiritsira Ntchito

1. Kupewa kuipitsidwa

• Ntchito zoyesera ziyenera kuchitika pamalo oyera, opanda fumbi, komanso m'malo oyera kwambiri kapena m'kabati yotetezeka yachilengedwe.
• Chepetsani nthawi yotsegula machubu okulirapo, ndipo ntchitoyi iyenera kuchitika mwachangu komanso motsatira malamulo kuti muchepetse chiopsezo chowonekera.
• Machubu odziyimira pawokha ayenera kugwiritsidwa ntchito pa mtundu uliwonse wa zitsanzo, ndipo kusakaniza n'koletsedwa kwambiri kuti kupewe kuipitsidwa ndi kusokoneza zotsatira za mayeso.

2. Kusunga ndi kugawa malo

• Musanagwiritse ntchito centrifugation, onetsetsani kuti chivundikiro cha screw chatsekedwa mwamphamvu kuti chisatuluke; komanso pangani mulingo wabwino mu centrifuge kuti mupewe kusalinganika kwa makina.
• Mukasunga, machubu ayenera kuyikidwa moyimirira kuti asatayike chifukwa cha malo opingasa. Pewani kuyika machubu pamalo otentha kwambiri, owala kwambiri kapena pamalo onyowa kuti musakhudze kukhazikika kwa zitsanzo ndi magwiridwe antchito a machubu.

3. Kusamalira zitsanzo zapadera

• Pa zitsanzo zomwe zili ndi zinthu zosungunuka, zachilengedwe kapena zinthu zowononga kwambiri, chitsanzo chapadera chomwe chili ndi kukana mankhwala chiyenera kugwiritsidwa ntchito.
• Kuti musunge zinthu zophwanyika, gwiritsani ntchito machubu osungira zinthu zophwanyika omwe sagwira ntchito kutentha kochepa; machubu wamba otungira zinthu omwe amatha kutayidwa amatha kusweka kapena kutayikira madzi kutentha kochepa kwambiri.

Zoyenera Kuchita Mukatha Kugwiritsa Ntchito

1. Kukonza chitetezo cha chilengedwe

• Machubu opangidwa ndi tizilombo toyambitsa matenda omwe ali ndi zitsanzo zamoyo zomwe zingapatsire matenda, zomwe zingakhale zoopsa kapena zoopsa kwambiri ayenera kuikidwa mu claved kapena kutsekedwa ndi mankhwala ophera tizilombo toyambitsa matenda mogwirizana ndi zofunikira za biosafety mu labotale asanatayidwe ngati zinyalala.
• Machubu otayidwa ayenera kuyikidwa mu chidebe cha zinyalala cha "pulasitiki yodetsedwa ndi zinthu zachilengedwe" malinga ndi dongosolo loyang'anira zinyalala zoopsa la labotale, ndipo sayenera kusakanizidwa ndi zinyalala wamba zotayidwa.

2. Malangizo okhudza zachilengedwe

• Ikani patsogolo machubu opangidwa ndi zinthu zobwezerezedwanso ndipo tengani nawo pulogalamu yobwezeretsanso zinthu ndi kutaya chilengedwe cha zinthu zogwiritsidwa ntchito m'ma laboratories komwe zinthu zimalola.
• Chepetsani kuwononga zinthu zosafunikira, limbikitsani kugwiritsa ntchito bwino zinthu zotayidwa popanda kugwiritsa ntchito molakwika poganizira za chitetezo, ndikulimbikitsa kumanga ma laboratories obiriwira.

Mafunso Ofunsidwa Kawirikawiri

1. Ndiyenera kuchita chiyani ngati sindingathe kulimbitsa chivundikiro chokulungira?

Choyamba onetsetsani kuti ulusi uli bwino komanso kuti palibe zinthu zakunja zomwe zakodwa mu ulusiwo. Musakakamize ulusiwo kuti utuluke chifukwa izi zitha kuwononga denga kapena chivundikirocho. Ngati sichikutsekabe, chubu chokulitsa chiyenera kusinthidwa ndi china chatsopano.

2. Kodi machubu otungira omwe angagwiritsidwe ntchito nthawi imodzi angagwiritsidwenso ntchito?

Kugwiritsa ntchito mobwerezabwereza sikuvomerezeka. Kutseka ndi kusabala kwa machubu otungira omwe angagwiritsidwe ntchito sikudzatsimikizika mutagwiritsa ntchito, ndipo kugwiritsanso ntchito kungayambitse kuipitsidwa, zotsatira zake zosayenera, kapena kuwonongeka kwa chubu.

3. Kodi ndiyenera kuchita chiyani ngati ndili ndi vuto la kutuluka madzi pamene ndikuyika centrifuging?

Onetsetsani kuti chivindikirocho chatsekedwa bwino ndipo chatsekedwa bwino, ndipo onetsetsani kuti machubu okulirapo ayikidwa bwino musanagwiritse ntchito centrifugation. Pewani kugwiritsa ntchito liwiro lopitirira la centrifugation lomwe machubuwo amayeretsedwa. Ngati kuli kofunikira, sankhani chubu chapadera cholimbana ndi kupanikizika ngati njira ina.

Mapeto

Kugwiritsa ntchito bwino machubu opangidwa ndi ulusi wotayidwa ndi sitepe yofunika kwambiri kuti zitsimikizire kulondola kwa zotsatira za mayeso, chitetezo cha zitsanzo ndi kudalirika kwa ntchito. Kutsatira kwambiri miyezo yogwirira ntchito ndi zofunikira za chitetezo cha chilengedwe, kaya pakusonkhanitsa zitsanzo, kusamalira, kusunga kapena kutaya, ndiye chitsimikizo chachikulu chokweza khalidwe la mayeso ndikuchepetsa zoopsa.

Alangizi a labu amalimbikitsa kuti akatswiri a labu agwiritse ntchito machubu apamwamba kwambiri okhala ndi kutseka bwino, kukana mankhwala komanso kutentha koyenera malinga ndi zosowa zawo zoyesera, kuti apititse patsogolo magwiridwe antchito a zoyeserera ndikukwaniritsa kayendetsedwe kokhazikika ka zinthu za labu.