Mawu Oyamba

M'ma laboratories amakono a biomedical ndi Chemical,machubu achikhalidwe otayika amagwiritsidwa ntchito kwambiri mu chikhalidwe cha ma cell, machitidwe amankhwala, kusungirako zitsanzo ndi ntchito zina zovuta.Kufunika kwawo muzoyesera sikungathe kunyalanyazidwa. Popeza machubu azikhalidwe awa amalumikizana mwachindunji ndi zitsanzo zoyesera, zinthu zake, kukula kwake, kutsekedwa komanso ngakhale atatsekeredwa kapena ayi zidzakhudza kwambiri zotsatira zoyeserera. Kusankhidwa kosayenera kungayambitse kuipitsidwa, kutayika kwa zitsanzo kapena kukondera kwa deta, motero kumakhudza kulondola ndi kuberekanso kwa phunzirolo.

Mitundu Yaikulu Ya Machubu Otayika Achikhalidwe

Pali mitundu yambiri yamachubu achikhalidwe omwe amatha kutaya, ndipo ochita kafukufuku ayenera kusankha mtundu woyenera malinga ndi cholinga cha kuyesa, malo ogwirira ntchito komanso mawonekedwe a zitsanzo. Iwo ali m'magulu atatu otsatirawa: zakuthupi, mphamvu ndi ntchito yapadera:

1. Kugawa ndi zinthu

Machubu azikhalidwe otayidwa opangidwa ndi zinthu zosiyanasiyana amasiyana pakukana kutentha, kukhazikika kwamankhwala ndi mawonekedwe a kuwala:

• Polypropylene: Kukana kwabwino kwa kutentha kwakukulu ndi dzimbiri la mankhwala, oyenera chikhalidwe cha ma cell, kuyesa kwa biology ndi ntchito zina.
• Polystyrene: mkulu transparency, zosavuta kuona madzi ndi selo boma, ambiri ntchito kuyezetsa kuwala, koma osati mkulu kutentha kukana, kawirikawiri sangagwiritsidwe ntchito autoclaving.
• Machubu opangira magalasi: Ngakhale kuti amatha kugwiritsidwanso ntchito komanso osasunthika pamankhwala, ndi okwera mtengo, amafunikira njira zina zoyeretsera ndi kutsekereza, ndipo amapereka chiwopsezo chotenga kachilomboka.

2. Kugawa ndi mphamvu

Kutengera kuchuluka kwa zitsanzo zomwe zimafunikira pakuyesa, kuchuluka kwa machubu achikhalidwe kumachokera ku yaying'ono mpaka ma voliyumu akulu:

• Machubu a Microcentrifuge: Zomwe zimagwiritsidwa ntchito popereka zitsanzo, mpweya wapakati, DNA/RNA m'zigawo ndi ntchito zina.
• Standard chikhalidwe machubu: mphamvu yomwe imagwiritsidwa ntchito kwambiri mu labotale, yoyenera chikhalidwe cha cell, kusakaniza zomwe zimachitika, kusunga zitsanzo ndi zolinga zina.
• Machubu a chikhalidwe chachikulu: oyenera chikhalidwe chachikulu cha cell kapena kuchuluka kwa njira zothetsera.

3. Gulu ndi ntchito yapadera

Machubuwa amapezeka ndi zina zowonjezera kuti akwaniritse zoyeserera zenizeni:

• Aseptic Pre-sterilized Tubes: fakitale yotsekedwa ndi ma radiation a gamma kapena autoclaving, yoyenera kuyesa ndi zofunikira zazikulu za aseptic.
• Ndi kapu ya cartridge: imalola kusinthana kwa gasi, koyenera tizilombo toyambitsa matenda kapena ma cell omwe amafunikira mpweya komanso kuteteza kuipitsidwa kuchokera kuzinthu zakunja.
• Machubu Osagwira Kutentha Kwambiri: Itha kugwiritsidwa ntchito mosamala mu -80 ℃ kapena ngakhale malo amadzimadzi a nayitrogeni, oyenera kusungirako kutentha kwanthawi yayitali kwa zitsanzo zachilengedwe.
• Machubu omaliza maphunziro / osamaliza maphunziro: Machubu omaliza maphunziro ndi osavuta kuwerengera mwachangu komanso kugawa kuchuluka kwamadzimadzi kuti muwonjezere luso lazoyeserera.

Zofunika Kwambiri Posankha Machubu Achikhalidwe

Popanga zoyeserera ndikugwiritsa ntchito, kusankha machubu oyenera otayirako ndikofunikira kuti zitsimikizire kuti zoyeserera ndi zolondola. Ochita kafukufuku ayenera kuganizira mozama zinthu zingapo zofunika:

1. Mtundu woyesera

Zofunikira zamachubu a chikhalidwe zimasiyana kwambiri kuyambira kuyesa mpaka kuyesa ndipo ziyenera kusankhidwa malinga ndi zomwe zikuchitika komanso malo oyesera:

• Za chikhalidwe cha ma cell: Zofunikira pa sterility ndizokwera kwambiri, ndipo kugwiritsa ntchito machubu osabala osabala omwe ali ndi zipewa za cartridge zopumira kuti zitsimikizire kusinthana kwa gasi.
• Kuyesa kwa PCR / molecular biology: ayenera kukhala opanda michere ya DNA, michere ya RNA ndi machubu opanda pyrogen, nthawi zambiri amagwiritsa ntchito machubu a polypropylene oyera.
• Kusungirako kutentha kochepa: Machubu okhala ndi kukana kwa kutentha kochepa ayenera kugwiritsidwa ntchito kupewa lysis pa kutentha kochepa.

2. Zitsanzo makhalidwe

Makhalidwe a physicochemical a chitsanzo amakhudza mwachindunji kusankha kwazinthu ndi kachitidwe kachitidwe ka machubu a chikhalidwe:

• Zitsanzo zamadzimadzi kapena zolimba: imatsimikizira kuchuluka kofunikira ndi mawonekedwe a chubu orifice.
• Zitsanzo za asidi kapena zamchere: Zitsanzo zowononga kwambiri zimafunikira zida zosagwirizana ndi mankhwala kuti zipewe kuwonongeka kwa chubu kapena kuipitsidwa.
• Kupewa kuwala: Zitsanzo zokhala ndi kuwala ziyenera kusankha amber kapena opaque chuma chikhalidwe chubu, kuteteza photodegradation.

3. Zofunika kulera

Kufunika kwa njira yolera yotseketsa komanso mtundu wa njira yotseketsa yomwe imagwiritsidwa ntchito ndizofunikira pakusankha machubu:

• Pre-sterilization vs: Zogulitsa zopangidwa ndi fakitale ndizoyenera kuyesa kwapamwamba, kupulumutsa nthawi; ngati labotale ili ndi zida zotsekereza za autoclave, mutha kusankha machubu a PP omwe atha kukhala opangidwa ndi autoclave.
• Kugwirizana kwa Sterilization: Mwachitsanzo, zinthu za PS sizoyenera ku autoclaving ndipo zimangogwiritsidwa ntchito kamodzi.

4. Kugwirizana

Machubu amayenera kusinthidwa bwino ndi zida za labotale kuti zitsimikizire kuti zikuyenda bwino komanso zotsatira zodalirika:

• Kugwirizana kwa Centrifugal: Machubu ogwiritsira ntchito centrifugation ayenera kukhala olimba kuti athe kupirira mphamvu zapakati pa liwiro lalikulu lozungulira.
• Kugwirizana kodzichitira: Pazoyeserera zomwe zimafuna kugwiritsa ntchito maloboti opaka mapaipi, makina opangira makina, ndi zina zotero, makulidwe achubu okhazikika ayenera kugwiritsidwa ntchito.

5. Mtengo ndi kukhazikika

Kuwongolera koyenera kwa ndalama ndi kugwiritsa ntchito zinthu ndizofunikiranso pakukwaniritsa zosowa zoyesera:

• Zotayika motsutsana ndi zogwiritsidwanso ntchito: machubu otayika ndi osavuta kuyendetsa ndikupewa kuipitsidwa, oyenerera kuyesa kwapamwamba; machubu agalasi ogwiritsidwanso ntchito ndi oyenera kuyesa koyambira ndi bajeti yochepa.
• Sikelo yogulira: Kugula kwakukulu kungachepetse mtengo wa unit, woyenera kwa nthawi yayitali, ntchito zazikulu; ang'onoang'ono, makonda okhazikika amakhala osinthika koma okwera mtengo.

Malangizo pa Zochitika Zomwe Mungagwiritsire Ntchito

Kutengera zosowa zamitundu yosiyanasiyana yoyesera, machubu azikhalidwe otayidwa otsatirawa amalimbikitsidwa pazochitika zingapo zomwe zimagwiritsidwa ntchito nthawi zambiri, ndicholinga chofuna kukonza bwino komanso kudalirika kwa zoyeserera:

1. Kulima ma cell

• Mtundu wovomerezeka: machubu osabala a polypropylene okhala ndi zipewa za cartridge zopumira
• Chifukwa: Zinthu za polypropylene zili ndi inertness yabwino yamankhwala komanso biocompatibility, yoyenera madzimadzi amtundu wa cell. Chophimba cha katiriji chimatha kuzindikira kusinthana kwa gasi, kuteteza kuipitsidwa ndi tizilombo tating'onoting'ono, ndikukwaniritsa malo omwe amafunikira kuti ma cell azichulukirachulukira.

2. PCR/qPCR kuzindikira kwa maselo

• Mitundu yovomerezeka: machubu a PCR opanda nyukiliya, opanda pyrogen kapena machubu a microcentrifuge
• Chifukwa: High chiyero polypropylene machubu, pambuyo mankhwala okhwima, angathe kupeweratu kuwononga zitsanzo kapena kuipitsidwa, kuonetsetsa zolondola ndi odalirika kakulidwe zotsatira. Mapangidwe a khoma la Thin-wall akulimbikitsidwa kuti apititse patsogolo kutentha.

3. Kusungirako kutentha kochepa

• Mtundu wovomerezeka: Machubu Osanja Kutentha Ochepa a Polypropylene okhala ndi Anti-Freeze Cracking Design ndi Screw Seal Cap
• Chifukwa: Machubuwa angagwiritsidwe ntchito kusungira zitsanzo kwa nthawi yaitali pa -80 ° C kapena ngakhale mumadzi a nayitrogeni, ndipo mawonekedwe apadera amalepheretsa chubu kuti lisawonongeke komanso kutayika kwachitsanzo. Oyenera kusungidwa kwa nthawi yayitali kwa mizere ya maselo, zitsanzo za magazi, mapuloteni kapena nucleic acid.

4. Centrifuge

• Mitundu yovomerezeka: machubu olekerera kwambiri a polypropylene, mapangidwe ozungulira kapena owoneka bwino pansi, okhala ndi zozungulira za centrifuge
• Chifukwa: Machubu a PP ali ndi kukana bwino kwa centrifugal ndipo amatha kupirira mphamvu zazikulu za centrifugal popanda kupunduka kapena kuphulika. Pansi pa conical imathandizira kusonkhanitsa ma cell kapena kutsika chapakati ndikuwonjezera kuchira.

Zolakwa Zodziwika ndi Njira Zopewera Izo

M'zochita, kulephera kuyesa chifukwa cha kusankha kosayenera kwa machubu achikhalidwe kumachitika nthawi ndi nthawi. Zolakwa zingapo zomwe zimadziwika bwino komanso malingaliro ofananirako a mayankho alembedwa pansipa kuti afotokozere ofufuza:

1. Kugwiritsa ntchito molakwika zinthu zosagwira kutentha kwambiri kwa autoclaving